The new restrictions impact U.S. passenger and recreational vessels, including cruise ships and yachts, and corporate and private aircraft.
The ban steers American dollars away from the Cuban regime and its military and security services, who control the tourism industry there.
Cruises from Port Canaveral to Cuba have been popular since 2016 when the Obama administration started allowing travel to the country.
"… We are adjusting the itineraries of our June 5 and June 6 sailings, which will no longer stop in Cuba," Royal Caribbean announced earlier this week.
The U.S. also bans most private planes and boats from going to Cuba. However, the Trump administration said it will allow anyone who has already paid for the trip to go ahead with it.
So-called "people to people" visits which allowed Americans to visit the island on cultural and educational tours are also no longer allowed.
But it appears university study-abroad activities are exempt from the travel ban.
UCF instructor Katie Coronado said she went to Cuba last summer with her students. She said she was given the green light to go again, but decided against it.
"I don't think that it's a good time to be there without freedom of speech, freedom of the press,” she said.