Petrus was a walk-on player at the University of Arkansas and awarded all-Southeastern Conference honors.
"We are deeply saddened by the passing of Mitch Petrus," the Arkansas football program said on social media Friday. "He was an outstanding competitor, incredible teammate and a true Hog. He will be greatly missed by many. Rest easy Mitch."
Petrus was drafted in the fifth round in 2010 by the Giants. He won a Super Bowl with them the following season. He briefly played with the New England Patriots and the Tennessee Titans his last season.
He retired in 2013 and moved back to central Arkansas.